Figure 7

Promoter methylation analysis of Cyclin D2. 7A-D: Medulloblastoma and astrocytoma cell lines unmethylation melting curve pattern (Figure 7A); hemi-methylation curve pattern (Figure 7B); partial methylation curve pattern (Figure 7C); and complete methylation curve (Figure 7D) of Cyclin D2 promoter shown by the MCA-Meth method. Figure 7E: Cyclin D2 promoter methylation in medulloblastoma cell lines by MSP. Three cell lines (Daoy, TE671c2 and D283) show hemimethylation (U+M) and the remaining three cell lines (TE671, PFSK-1 and SK-PN-DW) show complete methylation (M). Unmethylated and methylated PCR product bands are 222 and 276 bp, respectively. Figure 7F: Among astrocytic cell lines three of them (U87MG, SW1783 and GOS-3) show hemi-methylation; 2 show complete methylation (M) (T98G and CCF-STTG-1); and the other 2 (A172 and LN405) show no methylation (U). Figure 7G: Promoter methylation analysis of medulloblastoma samples. Figure 7H: Promoter methylation analysis of astrocytoma samples.