Protein expression of GLI1 and Cyclin D2 in astrocytic cell lines and tumor samples. Figure 5A: The first two cell lines (G1: U87MG and G2: A172) do not show very distinct bands, but the remaining 6 cell lines (G3: LN405, G4: SW1783, G5: T98G, G6: SW1088, G7: CCF-STTG-1 and G8: GOS-3) show distinct bands of GLI1 protein. Figures 5B and Figure 5C show GLI1 protein expression in astrocytic tumor samples 3-8 and 9, 10, 11, 20, 21 and 22. Figure 5D: Low protein expression of Cyclin D2 in astrocytic cell lines G1: U87MG, G2: A172, G3: LN405, G5: T98G, G6: SW1088, G7: CCF-STTG-1 and G8: GOS-3. Only one cell line (G4: SW1783) does not express Cyclin D2 protein. Figures 5E and Figure 5F: Cyclin D2 protein expression is not shown in astrocytic tumor samples (3-8 and 9, 10, 11, 20, 21 and 22).