Figure 4

qRT-PCR comparative expression of Cyclin D2, Plakoglobin, PAX6 and NKX2.2 in medulloblastoma and astrocytoma cell lines (for GLI1 and PTCH1, please check reference [26]). Figure 4A: All 6 medulloblastoma cell lines show equal expression of Cyclin D2 as compared to normal adult brain tissue; interestingly normal brain itself shows high expression of Cyclin D2. Figure 4B: Four astrocytic cell lines show very low expression and the remaining 4 cell lines show no expression of Cyclin D2 transcript as compared to normal adult brain tissue (p < 0.001). Figure 4C: All 6 medulloblastoma cell lines show high fold expression of Plakoglobin compared to normal adult brain tissue. Figure 4D: Five astrocytic cell lines show low expression of Plakoglobin transcript compared to normal brain tissue. Figure 4E: Only one medulloblastoma cell line, TE671c2, shows high expression fold of PAX6 as compared to normal adult brain tissue. Figure 4F: Most of the astrocytoma cell lines, except U87MG and LN405, show low expression of PAX6 transcript compared to normal brain tissue. Figure 4G: Most of the medulloblastoma cell lines show no/low expression of NKX2.2 except SK-PN-DW, as compared to normal adult brain tissue. Figure 4H: All 8 astrocytic cell lines show no/low expression of NKX2.2 transcript compared to normal brain tissue. SYBER Green dye was used for the quantification of cDNA by qRT-PCR. Comparative expression of samples was plotted on Y-axis with Log10.