qRT-PCR comparative expression of GLI1 , PTCH1, Cyclin D2, Plakoglobin, PAX6 and NKX2.2 in medulloblastoma and astrocytic primary tumor samples. Figure 3A: Most of the medulloblastoma primary tumor samples show high fold GLI1 transcript expression, but PTCH1 expression does not associate. Figure 3B: Most of the astrocytic primary tumor samples showed high fold GLI1 transcript expression but PTCH1 expression is low in most of the primary samples. Figure 3C: Most of the medulloblastoma samples show high expression of GLI1 and Cyclin D2. Figure 3D: No distinct pattern of Cyclin D2 expression in GBM samples. However, grade III astrocytoma samples show low expression in the presence of high GLI1 expression. Figure 3E: In case of Plakoglobin there is a reverse relationship in expression of GLI1 in medulloblastoma except samples 10 and 21. Figure 3F: Low-grade astrocytoma samples do not show Plakoglobin expression, and high-grade astrocytoma samples show Plakoglobin expression in the absence of GLI1 expression. Figure 3G: Reverse correlation between GLI1 and PAX6 transcript expression among medulloblastoma samples. Figure 3H: In the presence of high GLI1, there is low expression of PAX6 among astrocytic samples. Figure 3I: There is not any expression of NKX2.2 among medulloblastoma tumor samples. Figure 3J: Most of the astrocytic tumor samples show low/absence of expression of NKX2.2 in the presence of GLI1.