Figure 2

qRT-PCR comparative expression of GLI1 , PTCH1, Cyclin D2, Plakoglobin, PAX6 and NKX2.2 genes in GLI1 knock-down, negative and untransfected Daoy medulloblastoma cell line and U87MG astrocytoma cell line. After 72 h of siRNA-mediated transfection, GLI1 showed 83% decrease in expression in Daoy cell line (Figure 2A) and 40-45% decrease in expression in U87MG cell line (Figure 2B) compared to universal negative siRNA and untransfected cell lines. The siRNA-mediated GLI1 knock-down cell lines Daoy and U87MG showed 50% (Figure 2C) and 60% (Figure 2D) decrease in expression of PTCH1 respectively. The GLI1 knock-down cell lines (Daoy and U87MG) showed 17% decrease (Figure 2E) and 113% increase (Figure 2F) in expression of Cyclin D2 respectively. The cell lines Daoy and U87MG showed 30% decrease (Figure 2G) and 125% increase (Figure 2H) in expression of Plakoglobin respectively, compared to universal negative siRNA and untransfected cell lines. The knock-down cell lines (Daoy and U87MG) showed 35% decrease (Figure 2I) and 100% increase (Figure 2J) in expression of PAX6 respectively, compared to universal negative and untransfected cell lines. We further checked the expression of NKX2.2 in these two GLI1 knock-down cell lines (Daoy and U87MG) and found 50% decrease (Figure 2K) and not any changes in the expression of NKX2.2 (Figure 2L) respectively, compared to universal negative and untransfected cell lines.