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Figure 1 | BMC Cancer

Figure 1

From: Regulation of sonic hedgehog-GLI1 downstream target genes PTCH1, Cyclin D2, Plakoglobin, PAX6 and NKX2.2 and their epigenetic status in medulloblastoma and astrocytoma

Figure 1

Transfection of Daoy medulloblastoma cell line and U87MG astrocytoma cell line with GLI1 siRNA. Figure 1A: Daoy cell line is transfected with Stealth GLI1 siRNA (Invitrogen) with 100 pmol concentration, using Lipofectamine™ 2000 as a transfecting agent and fluorescein-labeled oligo (BLOCK-iT™ Fluorescent oligo) for identification of trasfection efficency. Figure 1B: Daoy cell line transfected with universal negative (U neg) siRNA. Figure 1C: Untransfected Daoy cell line. Figure 1D: U87MG cell line is transfected with Stealth GLI1 siRNA (Invitogen) with 100 pmol concentration, using Lipofectamine™ 2000 as a transfecting agent and fluorescein-labeled oligo (BLOCK-iT™ Fluorescent oligo) for identification of trasfection efficency. Figure 1E: U87MG cell line transfected with universal negative (U neg) siRNA. Figure 1F: Untransfected U87MG cell line.

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