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Figure 1 | BMC Cancer

Figure 1

From: Plexin-B1 silencing inhibits ovarian cancer cell migration and invasion

Figure 1

Expression of Plexin-B1 in benign, borderline, and malignant ovarian tissues and in ovarian cancer cell lines. Immunohistochemical staining showed the presence of Plexin-B1 in human ovarian tissues with different pathological changes. In normal ovaries (A) and benign serous ovarian cystadenomas (B), Plexin-B1 protein expression was virtually negative, whereas in serous borderline ovarian tumors, Plexin-B1 expression was higher and stained membranes; the insert shows the dashed line region at a higher magnification to demonstrate the membrane reaction product (C). In serous ovarian cystadenocarcinomas, Plexin-B1 expression was further up-regulated and showed strong cytoplasmic staining (D). Representative images are shown. Magnification, ×200. Expression of Plexin-B1 in four human ovarian cancer cell lines at both the mRNA and protein levels was detected with RT-PCR and western blotting. (E) RT-PCR showed the relative level of mRNA for Plexin-B1 in SKOV3, A2780, C13* and OV2008 cells. GAPDH was used for normalization. Product sizes: Plexin-B1, 150 bp; GAPDH, 240 bp. (F) Western blot showing Plexin-B1 protein expression in SKOV3, A2780, C13* and OV2008 cells. β-actin was used as a loading control. Product sizes: Plexin-B1, about 200 KD; β-actin, 43 KD.

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