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Table 1 Cell cycle analysis of SH-SY5Y cells treated with SI 34.

From: Antiproliferative and pro-apoptotic effects afforded by novel Src-kinase inhibitors in human neuroblastoma cells

cycle phase control SI 34 1 μM SI 34 10 μM Etoposide 50 μM
24 hours treatment
Sub G0 0 0 0 20 ± 1.2 ***
G0/G1 72 ± 1.7 81 ± 2.6 83 ± 1.8 * 69 ± 1.4
S 9 ± 0.5 4 ± 0.3 * 3 ± 0.7 * 3 ± 0.2 *
G2/M 19 ± 0.7 15 ± 1.0 14 ± 0.9  8 ± 0.8 *
48 hours treatment
Sub G0 0 12 ± 1.1 * 24 ± 2.2 *** 44 ± 2.7 ***
G0/G1 74 ± 3.1 76 ± 3.5 70 ± 3.6 48 ± 2.6 *
S 8 ± 0.8 4 ± 0.5 * 2 ± 0.1 * 1 ± 0.1 **
G2/M 18 ± 1.1 8 ± 1.1 * 4 ± 0.1 ** 7 ± 0.3 *
72 hours treatment
Sub G0 0 42 ± 3.2 *** 45 ± 2.9 *** 80 ± 4.1 ***
G0/G1 73 ± 4.7 58 ± 3.2 * 55 ± 2.97 * 20 ± 1.4 ***
S 11 ± 1.0 0 *** 0 *** 0 ***
G2/M 16 ± 0.8 0 *** 0 *** 0 ***
  1. Progression trough cell cycle was examined by flow cytometry analysis. Data analysis show that SI 34 arrests cell cycle at G0/G1 phase and abolishes the S and G2M phase. Etoposide 50 μM was used as a positive control for cell cycle arrest. Results are represented as percentages of the total cell population and are the mean ± S.E.M. of three independent experiments. *, ** and ***P < 0.05, P < 0.01 and P < 0.001 vs respective control