|
cycle phase
|
control
|
SI 34 1 μM
|
SI 34 10 μM
|
Etoposide 50 μM
|
|---|
|
24 hours treatment
|
|
Sub G0
|
0
|
0
|
0
|
20 ± 1.2 ***
|
|
G0/G1
|
72 ± 1.7
|
81 ± 2.6
|
83 ± 1.8 *
|
69 ± 1.4
|
|
S
|
9 ± 0.5
|
4 ± 0.3 *
|
3 ± 0.7 *
|
3 ± 0.2 *
|
|
G2/M
|
19 ± 0.7
|
15 ± 1.0
|
14 ± 0.9
|
8 ± 0.8 *
|
|
48 hours treatment
|
|
Sub G0
|
0
|
12 ± 1.1 *
|
24 ± 2.2 ***
|
44 ± 2.7 ***
|
|
G0/G1
|
74 ± 3.1
|
76 ± 3.5
|
70 ± 3.6
|
48 ± 2.6 *
|
|
S
|
8 ± 0.8
|
4 ± 0.5 *
|
2 ± 0.1 *
|
1 ± 0.1 **
|
|
G2/M
|
18 ± 1.1
|
8 ± 1.1 *
|
4 ± 0.1 **
|
7 ± 0.3 *
|
|
72 hours treatment
|
|
Sub G0
|
0
|
42 ± 3.2 ***
|
45 ± 2.9 ***
|
80 ± 4.1 ***
|
|
G0/G1
|
73 ± 4.7
|
58 ± 3.2 *
|
55 ± 2.97 *
|
20 ± 1.4 ***
|
|
S
|
11 ± 1.0
|
0 ***
|
0 ***
|
0 ***
|
|
G2/M
|
16 ± 0.8
|
0 ***
|
0 ***
|
0 ***
|
- Progression trough cell cycle was examined by flow cytometry analysis. Data analysis show that SI 34 arrests cell cycle at G0/G1 phase and abolishes the S and G2M phase. Etoposide 50 μM was used as a positive control for cell cycle arrest. Results are represented as percentages of the total cell population and are the mean ± S.E.M. of three independent experiments. *, ** and ***P < 0.05, P < 0.01 and P < 0.001 vs respective control
