Antiproliferative and pro-apoptotic effects afforded by novel Src-kinase inhibitors in human neuroblastoma cells

BMC Cancer

Table 1 Cell cycle analysis of SH-SY5Y cells treated with SI 34.

cycle phase	control	SI 34 1 μM	SI 34 10 μM	Etoposide 50 μM
*24 hours treatment*
Sub G0	0	0	0	20 ± 1.2 ***
G0/G1	72 ± 1.7	81 ± 2.6	83 ± 1.8 *	69 ± 1.4
S	9 ± 0.5	4 ± 0.3 *	3 ± 0.7 *	3 ± 0.2 *
G2/M	19 ± 0.7	15 ± 1.0	14 ± 0.9	8 ± 0.8 *
*48 hours treatment*
Sub G0	0	12 ± 1.1 *	24 ± 2.2 ***	44 ± 2.7 ***
G0/G1	74 ± 3.1	76 ± 3.5	70 ± 3.6	48 ± 2.6 *
S	8 ± 0.8	4 ± 0.5 *	2 ± 0.1 *	1 ± 0.1 **
G2/M	18 ± 1.1	8 ± 1.1 *	4 ± 0.1 **	7 ± 0.3 *
*72 hours treatment*
Sub G0	0	42 ± 3.2 ***	45 ± 2.9 ***	80 ± 4.1 ***
G0/G1	73 ± 4.7	58 ± 3.2 *	55 ± 2.97 *	20 ± 1.4 ***
S	11 ± 1.0	0 ***	0 ***	0 ***
G2/M	16 ± 0.8	0 ***	0 ***	0 ***

Progression trough cell cycle was examined by flow cytometry analysis. Data analysis show that SI 34 arrests cell cycle at G0/G1 phase and abolishes the S and G2M phase. Etoposide 50 μM was used as a positive control for cell cycle arrest. Results are represented as percentages of the total cell population and are the mean ± S.E.M. of three independent experiments. *, ** and ***P < 0.05, P < 0.01 and P < 0.001 vs respective control

ISSN: 1471-2407