Figure 4
From: Activated CD4+T cells enhance radiation effect through the cooperation of interferon-γ and TNF-α
Role of cytokines in the radiosensitization of tumor cells. On day -2, Hela cells were pre-treated for 2 days with IFN-γ; combination of 10 cytokines, IL-2, IL-4, IL-6, IL-8, IL-10, IL-12p40, IL-13, IFN-γ, TNF-α and GM-CSF, (Cyt); combination of cytokines without IFN-γ (Cyt-IFN); aCD4S; or combination of cytokines in the presence of neutralizing anti-IFN-γ antibody. On day 1, the cells were treated (or not) with 4 Gy γ-irradiation. Cells were plated in 96-well plate at 5 × 103 cells/well in 200 μl CM and cell growth inhibition was determined on day 5 by WST-1 assay. All experiments were performed in triplicate and repeated at least 2 times. Data represent mean values of each triplicate ± S.D.