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Figure 1 | BMC Cancer

Figure 1

From: Activated CD4+T cells enhance radiation effect through the cooperation of interferon-γ and TNF-α

Figure 1

Radiosensitizing effect of aCD4 on Hela cells. (A) γ-irradiation dose response curve. Hela cells were treated with various doses of γ-irradiation, plated at 5 × 103/well in 96-well plate containing 200 μl CM and cell viability was determined on day 5. (B, C, D) Suboptimal radiation dose of 4 Gy was chosen for subsequent experiments with aCD4 presensitization. On day -2, Hela cells were pre-sensitized with 1 × 104 aCD4 [aCD4 (1)] or 2 × 104 aCD4 [aCD4 (2)]. The cells were co-cultured directly with aCD4 (B), or via transwell system (C), or treated with conditioned medium (aCD4S) (D), followed (or not) on day 1 by 4 Gy γ-irradiation. Cell growth inhibition was determined on day 5 by WST-1 assay. All experiments were performed in triplicate and repeated at least 2 times. Data represent mean values of each triplicate ± S.D. Groups labeled ** (P < 0.01) were compared to irradiation alone group.

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