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Figure 6 | BMC Cancer

Figure 6

From: Role of 14-3-3σ in poor prognosis and in radiation and drug resistance of human pancreatic cancers

Figure 6

14-3-3σ over-expression increased drug-induced G2/M arrest and resistance to drug-induced apoptosis. A, Cell cycle distribution analysis. Vector- and 14-3-3σ-transfected stable MiaPaCa-2 cells were treated with 5 Gy γ-irradiation (IR), 10 nM mitoxantrone, or control without treatment followed by analysis of cell cycle distribution using FACS. B, Effect of 14-3-3σ radiation-induced apoptosis. Vector- and 14-3-3σ-transfected stable MiaPaCa-2 cells were treated with (filled bars) or without (white bars) γ-irradiation followed by analysis of cells at sub-G1 phase using FACS. C and D, Effect of 14-3-3σ on radiation- and drug-induced PARP cleavage. Vector- and 14-3-3σ-transfected stable MiaPaCa-2 cells were treated with 20 Gy γ-irradiation (C) or 10 μM mitoxantrone (D) for the times indicated followed by analysis of PARP cleavage using Western blot analysis. The graph in panel D shows the rate of production of the 85-kDa PARP fragments product (as a ratio of the 85-kDa fragment to the full-length 115-kDa protein).

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