Figure 5
From: Role of 14-3-3σ in poor prognosis and in radiation and drug resistance of human pancreatic cancers
14-3-3σ over-expression causes resistance to γ-irradiation and anticancer drugs in pancreatic cancer cell lines. A, Western blot analysis. Lysates from MiaPaCa-2 cells stably transfected with 14-3-3σ or vector control were prepared for Western blot analyses of 14-3-3σ and actin as a control. B, Effect of 14-3-3σ over-expression on radiation resistance. Clonogenic assay was used to determine the response of MiaPaCa-2 stable clones transfected with 14-3-3σ or vector control (Vec.) to γ-irradiation (IR). C, Effect of 14-3-3σ over-expression on drug resistance. SRB assay was used to determine the response of MiaPaCa-2 stable clones transfected with 14-3-3σ or vector control (Vec.) to anticancer drugs gemcitabine (GEM), mitoxantrone (MX), and Adriamycin (Adr). RRF = relative resistance factor derived by dividing the IC50 of 14-3-3σ-expressing cells by that of the vector-transfected control cells. D, Western blot analysis. Lysates from BxPc-3 cells stably transfected with 14-3-3σ or scrambled shRNA control were prepared for Western blot analyses of 14-3-3σ and actin as a control. E and F, Effect of 14-3-3σ knockdown on treatment resistance. SRB assay was used to determine the response of BxPc-3 stable clone transfected with 14-3-3σ or scrambled (Scr) shRNA control to γ-irradiation and anticancer drugs. RRF = relative resistance factor derived by dividing the IC50 of 14-3-3σ-knockdown cells by that of the scrambled control cells. (* = p value < 0.05; ** = p value < 0.01).