Relationship between 14-3-3σ expression and drug resistance of pancreatic cancer cell lines. A, Expression level of 14-3-3σ in BxPc-3 and MiaPaCa-2 cells. Lysates from BxPc-3 or MiaPaCa-2 cells were prepared for Western blot analyses of 14-3-3σ and actin as a loading control. B, C, and D, Correlation of 14-3-3σ expression and treatment resistance. The effect of γ-irradiation (B) and anticancer drugs Adriamycin (C) and mitoxantrone (D) on the survival of BxPc-3 and MiaPaCa-2 cells was determined using SRB assay. The survival following treatments was normalized to the control without irradiation or drug treatment.