Correlation of 14-3-3σ expression in pancreatic cancer tissues with lymph node metastasis. Thirty-eight fresh frozen human pancreatic ductal adenocarcinoma tissues with (+) or without (-) lymph node (LN) metastasis were analyzed using Western blot for the protein level of 14-3-3σ and GAPDH control. The relative level (R.L.) of 14-3-3σ in each sample was measured and normalized to that of GAPDH. In each gel, lysates from BxPc-3 (BxPc) cells were used as a control and the level of 14-3-3σ in BxPc-3 cells was set to 1.