Figure 1

U87 cell proliferation assay. A. The WST-1 assay was performed to measure U87 cell viability after treatment with increasing concentrations of Res (DEMSO, 0, 1, 10, and 100 μM, and 1 mM) for different exposure times (0, 24, 48, or 72 h). The groups treated with 100 μM Res for 48 and 72 h showed the greatest significant difference compared with the other groups (P < 0.01). Asterisks indicate statistically significant changes (P < 0.01) after Res treatment. Bars indicate SE. B. The trypan blue dye exclusion assay revealed the U87 cell viability after treatment with increasing concentrations of Res (0, 1, 10, and 100 μM, and 1 mM) for 48 h. Asterisks indicate statistically significant changes (P < 0.01) after Res treatment. Bars indicate SE. C. Morphology of U87 cells treated by 100 μM Res for 48 h under a light microscope (a.b); Morphology of U87 cells treated by 100 μM Res for 48 h in trypan blue dye cell exclusion assay (c.d). Original magnification: 40×.