Figure 8

SP-DiI labeled rat UCMSCs administered intratracheally were detected near the tumors. This experiment suggests that intratracheally administered rat UCMSCs home at adjacent to the lung tumor area. Serial sections from paraffin-embedded lung were used for the detection of rat UCMSCs labeled with SP-DiI fluorescent dye either with SP-DiI fluorescence alone (B-i) or with both DAPI nuclear staining and SP-DiI fluorescence (B-ii). Tumor morphology was observed after H&E staining (A, PBS-treated tumors, arrows indicate tumors, and B-iii, rat UCMSC-treated tumor, arrow indicates tumor margin). Scale bars in Panel A and B indicate 500 μm and 100 μm, respectively.