Figure 6

Rat UCMSCs significantly attenuated protein expression of both cyclin A (Panels A and B) and CDK2 (Panels A and C), but not cyclin E (Panel A), in co-cultured LLC cells. This experiment suggests that indirect co-culture of a small number of rat UCMSCs caused G0/G1 arrest in LLC cells. Samples were prepared as described in the Figure 5 legend and in Materials and Methods, and subjected to Western blot analysis. Sample preparation and Western blot analysis were performed three times with duplicate determinations. The pictures (Panel A) represent typical blotting results. P values were calculated as compared to the level of control.