Figure 5
Indirect co-culture of a small number of rat UCMSCs with LLC cells in a Transwell cell culture caused G0/G1 arrest in LLC cells. In the co-culture study, 1.67 × 104 or 3.33 × 104 rat UCMSCs were placed in the culture plate inserts and co-cultured with 1 × 105 LLC cells in the bottom of the culture plates. After two to three days co-culture, LLC cells were collected from the bottom chamber by trypsinization and subjected to flow cytometry as described in Materials and Methods. Panel A represents the flow cytometry result and panel B represents the histogram pattern of different phases of the cell cycle. The experiment was performed twice with triplicate determinations.