Defensin alpha 6 (DEFA 6) overexpression threshold of over 60 fold can distinguish between adenoma and fully blown colon carcinoma in individual patients

Table 2 Sequence of oligonucleotide primers used for PCR amplification and product size predicted for sample cDNA.

Gene name	Gene annotation	RefSeq ID	Sequence	Amplicon size
DEFA 1-3*	Defensin, alpha 1 to 3	NM_005218.3	CCTGCCTAGCTAGAGGATCTGTG	222 bp
		NM_005217.2	TGTTTTTCCTTGAGCCTGGA
DEFA 6*	Defensin, alpha 6, Paneth cell-specific	NM_001926.2	CTCAAGTCTTAGAGCTTTGGGCT	198 bp
			GGACACACGACAGTTTCCTTC
β-actin*	Beta actin	NM_001101	AGAGCCTCGCCTTTGCCGAT	160 bp
			CCCACGATGGAGGGGAAGAC

ISSN: 1471-2407