Blocking IKK activity results in TRAIL-induced apoptosis in A549 cells. FITC-conjugated Annexin V and Propidium Iodide (PI) staining were utilized as apoptosis indicators following infection of A549 lung cancer cells with various combinations of adenovirus constructs as stated. 104 A549 lung cancer cells were gated for each histogram. Histograms are depicted in two different panels for clarity. AdIKKβKA and Ad5hTRAIL constructs were used in 5,000 DNA particles/cell unless stated otherwise. AdCMVLacZ construct was used at an MOI of 10,000 DNA particles/cell. Uninfected but FITC-Annexin V and PI stained A549 lung cancer cells were used as controls (gray line).