Figure 6

Blocking IKK activity results in TRAIL-induced apoptosis in A549 cells. FITC-conjugated Annexin V and Propidium Iodide (PI) staining were utilized as apoptosis indicators following infection of A549 lung cancer cells with various combinations of adenovirus constructs as stated. 10⁴ A549 lung cancer cells were gated for each histogram. Histograms are depicted in two different panels for clarity. AdIKKβKA and Ad5hTRAIL constructs were used in 5,000 DNA particles/cell unless stated otherwise. AdCMVLacZ construct was used at an MOI of 10,000 DNA particles/cell. Uninfected but FITC-Annexin V and PI stained A549 lung cancer cells were used as controls (gray line).