Figure 4

Evidence indicates the interaction of GSK-3β and PMS2 in HeLa cells. (A) Representative result of IP and immunobloting was presented after GSK-3β WT plasmid transfection. Interaction of GSK-3β and PMS2 in HeLa cells was suggested by using IP with an antibody to GSK-3β and immunoblotting with an antibody to PSM2; (B) Representative result of immunobloting was presented after GSK-3β plasmid transfection and Lithium administration; (C) GSK-3β significantly enhanced PMS2 production in HeLa cells, which was reversed by 20 nm of Lithium administration, * P < 0.05, compared to the control cells; ** P < 0.05, compared to the transfected cells; (D) Representative result of immunobloting was presented after GSK-3β plasmid transfection and RNA interference; (E) GSK-3β siRNA significantly reversed GSK-3β-enhanced PMS2 production in HeLa cells in a concentration-dependent fashion, * P < 0.05, compared to the control cells; (F) PMS2 protein was downregulated significantly after GSK-3β siRNA administration, showing a dose-dependent correlation (r = 0.792, P < 0.05).