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Figure 6 | BMC Cancer

Figure 6

From: Epigenetic inactivation of the NORE1gene correlates with malignant progression of colorectal tumors

Figure 6

Suppression of tumor cell growth by NORE1A and NORE1B. Transient transfection of expression vectors encoding WT-NORE1A and WT-NORE1B in Caco-2 cells (A). A dose-associated expression of NORE1A and NORE1B was determined at 72 h post-transfection using semi-quantitative RT-PCR. Suppression of Caco-2 cell growth by NORE1A or NORE1B (B). Caco-2 cells were transfected with 2 μg of WT-NORE1A or WT-NORE1B and cell numbers were counted at 24 h intervals. The assay was performed in triplicate (*, P < 0.05). SiRNA-mediated knockdown of NORE1 expression in HCT116 cells (C). The cells were transfected with increasing doses of siRNA and NORE1A and NORE1B levels were determined by RT-PCR at 72 h post-transfection. Anti-proliferative role of endogenous NORE1A and NORE1B (D). HCT116 cells were transfected with 50 nM of siNORE1A, siNORE1B or siControl and cell numbers were counted at 24 h intervals. The assay was performed in triplicate (*, P < 0.05). Suppression of colony formation of tumor cells by NORE1 (E). Caco-2 and HCT116 cells transfected with 2 μg of expression vectors or siRNA were maintained in the presence of G418 (400 ng) for 7 days. The assay was performed in triplicate (*, P < 0.05; **, P < 0.01).

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