Skip to main content
Figure 4 | BMC Cancer

Figure 4

From: Epigenetic inactivation of the NORE1gene correlates with malignant progression of colorectal tumors

Figure 4

Genomic status of NORE1 and re-activation by 5-Aza-dC. Genomic level of NORE1 in colorectal cancer cell lines (A). The NORE1 gene was amplified by genomic PCR using intron-specific primer sets. Ten μl of the PCR products were resolved on a 2% agarose gel. GAPDH was used as an endogenous control. Comparison of NORE1 gene level between tumor and adjacent noncancerous tissues (B). Effect of 5-Aza-dC and TSA on NORE1A and NORE1B mRNA expression (C). Caco-2 and Colo320 cells were treated with increasing doses of 5-Aza-dC for 4 days. For combined treatment with TSA, the cells were incubated with 5-Aza-dC for 48 h and then exposed to TSA (250 ng/ml) for 48 h. Elevation of NORE1A and NORE1B mRNA expression in low expressor cell lines following 5-Aza-dC treatment (D). Cancer cell lines with low NORE1 level were treated with 5-Aza-dC (5 μM) for 5 days and NORE1A and NORE1B expression was evaluated by RT-PCR analysis. C, untreated control; T, 5-Aza-dC treated.

Back to article page