Table 1 Primers used in the study
Oligonucleotide1 | Localization2 | Sequence3 | Screening |
|---|---|---|---|
tert e3-M331s | e3 (2210-39) | CTGTTCCTGCCTATGAACATTGTTACCGTG | chTERT cDNA |
tert e3M137-s | e3 (2260-80) | CTATACTGGCTGATGGATTCC | exon 4 |
M139s | e4 (2480-500) | CATCAAGGCTCCGGTTCATTC | exon 5 |
M141s | e5 (2680-700) | GGGAGAGATGATATCTACAGG | exon 6 |
M142r | e5 (2700-680) | CCTGTAGATATCATCTCTCCC | exon 4 |
M143s | e6 (2815-35) | GTGGAAGTGATATCACAGGTC | exon 7 |
M144r | e6 (2835-15) | GACCTGTGATATCACTTCCAC | exon 5 |
M145s | e7 (2974-92) | GTGTCCAAGCTTCAAGAGA | exon 8 |
M146r | e7 (2992-74) | TCTCTTGAAGCTTGGACAC | exon 6 |
M147s | e8 (3040-60) | AATGAGAACAGTTCCACCCTG | exon 9 |
M148r | e8 (3060-40) | CAGGGTGGAACTGTTCTCATT | exon 7 |
M149s | e9 (3172-92) | AGCTTATGCTACGGAGACATG | exon 10 |
M150r | e9 (3192-72) | CATGTCTCCGTAGCATAAGCT | exon 8 |
M151s | e10 (3258-79) | GCTGGTTACGCCACATTTAATG | exon 11 |
M152r | e10 (3279-58) | CATTAAATGTGGCGTAACCAGC | exon 9 |
M153s | e11 (3373-93) | GATGATATCCCGGGATGTTCC | exon 12 |
M154r | e11 (3393-73) | GGAACATCCCGGGATATCATC | exon 10 |
M155s | e12 (3556-76) | TGCAAATTGACTGCAGTCCTC | exon 13 |
rM156r | e12 (3576-56) | GAGGACTGCAGTCAATTTGCA | exon 11 |
M157s | e13 (3618-42) | CAGCCTTCAGACAGTTCTAATTAAC | exon 14 |
M158r | e13 (3642-18) | GTTAATTAGAACTGTCTGAAGGCT | exon 12 |
M159s | e14 (3727-47) | CCTGATTTCTTCCTAAGGATC | exon 15 |
M160r | e14 (3747-27) | GATCCTTAGGAAGAAATCAGG | exon 13 |
M162r | e15 (3870-50) | ATGGTAGCACAGCCATTCTGC | exon 14 |
M163r | e16 (3996-76) | CACCGTCTTCAGCAGTTCCAT | exon 15 |
tert e16-M316-r | e16 (4041-07) | TTAGTCCAGTATAGTTTTGAAATCTTGACAAAGCG | chTERT cDNA |
TET-tert e4 | e4 (2554-78) | TET-CAGAAACTCAGCAAGGAAAGCAGAG | splice variant 5 |
tert e6 | e6 (2874-55) | CCACCTTATTCCATAGACAG | splice variant 5 |
TET-tert e9 | e9 (3170-92) | TET-GCAGCTTATGCTACGGAGACATG | splice variant 10 |
tert e11 | e11 (3361-42) | TCACCACAGTCTTCTTGGCA | splice variant 10 |