Figure 4

Regulation of chTERT splicing involving variants 5 and 10 during lymphomagenesis in vivo. Proportions of constitutively spliced chTERT transcript (in blue) and alternatively spliced variants d10f+i10ec (in yellow) and the d5f variant (in green), as shown in the panels on the right and left, respectively. The proportions correspond to the peak area obtained by capillary electrophoresis analysis of PCR targeting exon 5 (A) or 10 (B), performed on cDNA extracted from sorted CD4⁺ T cells sampled from GaHV-2-infected chickens at 5 different time points after infection, as indicated on the x-axis. The curve shows telomerase activity, which was obtained by summing the peak areas corresponding to the elongation products (right y-axis) (C) Proportions of d10f (green) and i10ec (orange) variants, as indicated above the graph and corresponding to 100% of alternative splice variants10 at each time point after infection, as shown in (B).