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Table 1 Effects of test substances on proliferation of certain cancer cells.

From: Protection of p53 wild type cells from taxol by nutlin-3 in the combined lung cancer treatment

The distribution of cells in different cell cycle phases (%)1
  A549 FaDu H1299
  <2C2 G0 + 1 S G2 + M <2C G0 + 1 S G2 + M <2C G0 + 1 S G2 + M
5-fluorouracil (μM)
1 2 ± 1 62 ± 2 27 ± 1 11 ± 1 2 ± 1 51 ± 3 31 ± 2 16 ± 1 2 ± 1 56 ± 2 31 ± 2 15 ± 1
3 2 ± 1 57 ± 2 33 ± 1 10 ± 1 2 ± 1 55 ± 3 26 ± 2 19 ± 1 2 ± 1 52 ± 2 34 ± 2 14 ± 1
10 2 ± 1 31 ± 2 61 ± 2 8 ± 2 2 ± 1 28 ± 1 52 ± 1 20 ± 1 2 ± 1 52 ± 2 32 ± 2 16 ± 2
30 3 ± 1 46 ± 2 47 ± 2 7 ± 2 3 ± 1 48 ± 2 44 ± 1 18 ± 1 5 ± 1 50 ± 2 33 ± 2 17 ± 2
Camptothecin (nM)
10 2 ± 1 42 ± 4 24 ± 2 28 ± 3 6 ± 2 18 ± 1 24 ± 2 58 ± 3 2 ± 1 17 ± 4 40 ± 9 43 ± 9
30 8 ± 2 16 ± 2 68 ± 4 16 ± 4 10 ± 3 6 ± 1 77 ± 3 17 ± 3 12 ± 2 11 ± 3 69 ± 3 19 ± 2
100 32 ± 4 14 ± 2 69 ± 4 17 ± 2 17 ± 2 21 ± 3 68 ± 4 11 ± 1 17 ± 3 22 ± 1 56 ± 4 22 ± 3
300 45 ± 6 45 ± 4 45 ± 4 10 ± 2 15 ± 3 40 ± 3 50 ± 3 10 ± 2 15 ± 2 35 ± 3 40 ± 4 25 ± 2
Nutlin-3 (μM)
1 2 ± 1 61 ± 2 28 ± 2 11 ± 1 2 ± 1 40 ± 4 38 ± 3 22 ± 3 2 ± 2 57 ± 1 29 ± 2 14 ± 1
3 2 ± 1 71 ± 2 13 ± 1 16 ± 1 2 ± 1 44 ± 2 34 ± 2 22 ± 2 2 ± 1 57 ± 3 25 ± 2 18 ± 2
10 2 ± 1 69 ± 1 7 ± 3 24 ± 3 4 ± 1 45 ± 3 34 ± 2 21 ± 2 4 ± 1 54 ± 4 27 ± 4 19 ± 2
30 5 ± 1 68 ± 2 6 ± 1 26 ± 2 8 ± 1 48 ± 3 32 ± 2 20 ± 2 7 ± 1 63 ± 2 19 ± 2 18 ± 1
Roscovitine (μM)
1 2 ± 1 60 ± 2 24 ± 2 16 ± 1 2 ± 1 41 ± 4 39 ± 4 20 ± 2 2 ± 1 47 ± 1 36 ± 3 17 ± 2
3 2 ± 1 59 ± 4 20 ± 3 21 ± 1 2 ± 1 43 ± 1 32 ± 1 25 ± 1 2 ± 1 52 ± 1 33 ± 1 15 ± 2
10 5 ± 1 59 ± 4 20 ± 3 21 ± 1 4 ± 1 38 ± 2 27 ± 2 35 ± 2 4 ± 2 49 ± 2 29 ± 1 22 ± 1
30 10 ± 4 51 ± 5 24 ± 3 24 ± 3 10 ± 3 34 ± 3 30 ± 2 36 ± 2 11 ± 4 43 ± 3 26 ± 2 31 ± 3
Taxol (nM)
1 9 ± 2 60 ± 4 25 ± 4 15 ± 2 21 ± 2 39 ± 3 34 ± 6 27 ± 4 13 ± 6 48 ± 6 30 ± 3 22 ± 5
3 36 ± 5 41 ± 3 32 ± 5 27 ± 2 30 ± 5 22 ± 4 35 ± 5 43 ± 7 35 ± 6 40 ± 1 40 ± 1 20 ± 1
10 35 ± 6 22 ± 4 29 ± 3 51 ± 6 33 ± 6 12 ± 2 19 ± 4 69 ± 4 42 ± 5 34 ± 4 34 ± 4 32 ± 7
30 16 ± 4 15 ± 2 17 ± 3 68 ± 5 16 ± 3 4 ± 1 11 ± 2 85 ± 2 44 ± 4 18 ± 1 36 ± 4 46 ± 7
100 10 ± 2 18 ± 4 18 ± 3 64 ± 5 8 ± 2 5 ± 1 9 ± 3 87 ± 3 30 ± 4 21 ± 3 23 ± 3 55 ± 2
Control
- 3 2 ± 1 62 ± 1 26 ± 1 12 ± 1 2 ± 1 46 ± 2 36 ± 1 18 ± 1 2 ± 1 59 ± 2 26 ± 2 15 ± 1
  1. The data show the effects of the test substances on the cell cycle of A549, FaDu and H1299 cells after exposure for 1 day (average ± SD of 6 independent experiments). Significant differences (p < 0.05) to control cultures are indicated in bold print.
  2. 1The distribution of cells in different cell cycle phases was calculated from the population of cycling cells (G0 + 1 + S + G2 + M = 100%).
  3. 2The percentage of hypodiploid cells (<2C DNA content) was calculated from the total number of cells.
  4. 3Since the test compounds were dissolved in a DMSO stock solution, control cells cultures were made in 14 mM (0.1%) DMSO.
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BMC Cancer

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