From: Protection of p53 wild type cells from taxol by nutlin-3 in the combined lung cancer treatment
The distribution of cells in different cell cycle phases (%)1 | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
A549 | FaDu | H1299 | ||||||||||
<2C2 | G0 + 1 | S | G2 + M | <2C | G0 + 1 | S | G2 + M | <2C | G0 + 1 | S | G2 + M | |
5-fluorouracil (μM) | ||||||||||||
1 | 2 ± 1 | 62 ± 2 | 27 ± 1 | 11 ± 1 | 2 ± 1 | 51 ± 3 | 31 ± 2 | 16 ± 1 | 2 ± 1 | 56 ± 2 | 31 ± 2 | 15 ± 1 |
3 | 2 ± 1 | 57 ± 2 | 33 ± 1 | 10 ± 1 | 2 ± 1 | 55 ± 3 | 26 ± 2 | 19 ± 1 | 2 ± 1 | 52 ± 2 | 34 ± 2 | 14 ± 1 |
10 | 2 ± 1 | 31 ± 2 | 61 ± 2 | 8 ± 2 | 2 ± 1 | 28 ± 1 | 52 ± 1 | 20 ± 1 | 2 ± 1 | 52 ± 2 | 32 ± 2 | 16 ± 2 |
30 | 3 ± 1 | 46 ± 2 | 47 ± 2 | 7 ± 2 | 3 ± 1 | 48 ± 2 | 44 ± 1 | 18 ± 1 | 5 ± 1 | 50 ± 2 | 33 ± 2 | 17 ± 2 |
Camptothecin (nM) | ||||||||||||
10 | 2 ± 1 | 42 ± 4 | 24 ± 2 | 28 ± 3 | 6 ± 2 | 18 ± 1 | 24 ± 2 | 58 ± 3 | 2 ± 1 | 17 ± 4 | 40 ± 9 | 43 ± 9 |
30 | 8 ± 2 | 16 ± 2 | 68 ± 4 | 16 ± 4 | 10 ± 3 | 6 ± 1 | 77 ± 3 | 17 ± 3 | 12 ± 2 | 11 ± 3 | 69 ± 3 | 19 ± 2 |
100 | 32 ± 4 | 14 ± 2 | 69 ± 4 | 17 ± 2 | 17 ± 2 | 21 ± 3 | 68 ± 4 | 11 ± 1 | 17 ± 3 | 22 ± 1 | 56 ± 4 | 22 ± 3 |
300 | 45 ± 6 | 45 ± 4 | 45 ± 4 | 10 ± 2 | 15 ± 3 | 40 ± 3 | 50 ± 3 | 10 ± 2 | 15 ± 2 | 35 ± 3 | 40 ± 4 | 25 ± 2 |
Nutlin-3 (μM) | ||||||||||||
1 | 2 ± 1 | 61 ± 2 | 28 ± 2 | 11 ± 1 | 2 ± 1 | 40 ± 4 | 38 ± 3 | 22 ± 3 | 2 ± 2 | 57 ± 1 | 29 ± 2 | 14 ± 1 |
3 | 2 ± 1 | 71 ± 2 | 13 ± 1 | 16 ± 1 | 2 ± 1 | 44 ± 2 | 34 ± 2 | 22 ± 2 | 2 ± 1 | 57 ± 3 | 25 ± 2 | 18 ± 2 |
10 | 2 ± 1 | 69 ± 1 | 7 ± 3 | 24 ± 3 | 4 ± 1 | 45 ± 3 | 34 ± 2 | 21 ± 2 | 4 ± 1 | 54 ± 4 | 27 ± 4 | 19 ± 2 |
30 | 5 ± 1 | 68 ± 2 | 6 ± 1 | 26 ± 2 | 8 ± 1 | 48 ± 3 | 32 ± 2 | 20 ± 2 | 7 ± 1 | 63 ± 2 | 19 ± 2 | 18 ± 1 |
Roscovitine (μM) | ||||||||||||
1 | 2 ± 1 | 60 ± 2 | 24 ± 2 | 16 ± 1 | 2 ± 1 | 41 ± 4 | 39 ± 4 | 20 ± 2 | 2 ± 1 | 47 ± 1 | 36 ± 3 | 17 ± 2 |
3 | 2 ± 1 | 59 ± 4 | 20 ± 3 | 21 ± 1 | 2 ± 1 | 43 ± 1 | 32 ± 1 | 25 ± 1 | 2 ± 1 | 52 ± 1 | 33 ± 1 | 15 ± 2 |
10 | 5 ± 1 | 59 ± 4 | 20 ± 3 | 21 ± 1 | 4 ± 1 | 38 ± 2 | 27 ± 2 | 35 ± 2 | 4 ± 2 | 49 ± 2 | 29 ± 1 | 22 ± 1 |
30 | 10 ± 4 | 51 ± 5 | 24 ± 3 | 24 ± 3 | 10 ± 3 | 34 ± 3 | 30 ± 2 | 36 ± 2 | 11 ± 4 | 43 ± 3 | 26 ± 2 | 31 ± 3 |
Taxol (nM) | ||||||||||||
1 | 9 ± 2 | 60 ± 4 | 25 ± 4 | 15 ± 2 | 21 ± 2 | 39 ± 3 | 34 ± 6 | 27 ± 4 | 13 ± 6 | 48 ± 6 | 30 ± 3 | 22 ± 5 |
3 | 36 ± 5 | 41 ± 3 | 32 ± 5 | 27 ± 2 | 30 ± 5 | 22 ± 4 | 35 ± 5 | 43 ± 7 | 35 ± 6 | 40 ± 1 | 40 ± 1 | 20 ± 1 |
10 | 35 ± 6 | 22 ± 4 | 29 ± 3 | 51 ± 6 | 33 ± 6 | 12 ± 2 | 19 ± 4 | 69 ± 4 | 42 ± 5 | 34 ± 4 | 34 ± 4 | 32 ± 7 |
30 | 16 ± 4 | 15 ± 2 | 17 ± 3 | 68 ± 5 | 16 ± 3 | 4 ± 1 | 11 ± 2 | 85 ± 2 | 44 ± 4 | 18 ± 1 | 36 ± 4 | 46 ± 7 |
100 | 10 ± 2 | 18 ± 4 | 18 ± 3 | 64 ± 5 | 8 ± 2 | 5 ± 1 | 9 ± 3 | 87 ± 3 | 30 ± 4 | 21 ± 3 | 23 ± 3 | 55 ± 2 |
Control | ||||||||||||
- 3 | 2 ± 1 | 62 ± 1 | 26 ± 1 | 12 ± 1 | 2 ± 1 | 46 ± 2 | 36 ± 1 | 18 ± 1 | 2 ± 1 | 59 ± 2 | 26 ± 2 | 15 ± 1 |