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Table 4 Effects of silencing RASSF1C on target genes

From: Ras-association domain family 1C protein promotes breast cancer cell migration and attenuates apoptosis

Gene

Fold change

RASSF1C

-5

Caspase 3

+1.3

CXCR4

-2

  1. Caspase3 and CXR4 gene expression in T47D cells treated with Mission® lentiviral transduction particles specific to RASSF1C. The fold change is compared to T47D cells treated control scrambled shRNA (Mission® lentiviral transduction control particles). The RT-PCR reactions were carried out in triplicate, and the fold change was calculated using the 2-ΔΔCT method [22]. Cyclophyllin was used as a loading control.