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Table 1 RASSF1A and RASSF1C mRNA expression in epithelial and breast cancer cell lines

From: Ras-association domain family 1C protein promotes breast cancer cell migration and attenuates apoptosis

Cell Line Gene Relative expression levels
AG1132B RASSF1A 1
  RASSF1C 1
Hs578T RASSF1A -3.3
  RASSF1C 4
MDA-MB231 RASSF1A -2.2
  RASSF1C 7
T47D RASSF1A -8
  RASSF1C 6
  1. QRT-PCR analysis of total RNA from human primary mammary epithelial cells (AG1132B) and from established breast cancer cell lines, Hs578T. MDA-MB231, and T47D cells. 1 μg of total RNA was used to perform reverse transcription reactions (RTs) and 1 ul of the RT reaction was used to set up qRT-PCR reactions in triplicates using RASSF1A and RASSF1C specific primers. All qRT-PCR reactions were set in triplicates and Cyclophillin was used as an internal loading control and used to normalize the relative expression levels using the 2-ΔΔ method [42]. The RT-PCR analysis shows that RASSF1A expression was down and RASSF1C expression is up in established breast cancer cell lines compared the primary mammary epithelial cells.