Figure 3

(A) Western blot analysis of caspase 3, CXCR4, GHR, and TGM2 expression in T47D cells stably transduced with empty MLV-backbone (T47D-BB) or MLV-HA-RASSF1C (T47D-1C) using their corresponding antibodies. The T47D cells were treated with 1 μg/ml doxycycline for 48 hr and used for Western blot analysis. It is clear that caspase 3 and TGM2 protein levels are down regulated and CXCR4 and GHR are up regulated in T47D cells over expressing RASSF1C validating the microarray and qRT-PCR findings (Table 1 and 2). Activation of ERK1/2 (B) and total ERK1/2 levels (C) were assessed in T47D-1C cells. The level of phosphorylated ERK1/2 in T47D-1C is much higher compared to that in T47D-BB while the total ERK1/2 protein levels are relatively the same in T47D-BB and T47D-1C.