Figure 5

Combination of Sorafenib with cytotoxic drugs inhibits cell proliferation and induces apoptosis and necrosis. SEM cells were treated both separately and in combination with Sorafenib (0.73 μM or 7.3 μM) and with cytarabine (250 nM) or doxorubicin (12.5 nM) or RAD001 (1 nM or 10 nM) for 72 h. All drugs had effects on proliferation and rate of apoptosis and necrosis between treatment groups. Statistically significant effects were detected in regards to proliferation between all combination treatments vs. DMSO control and in regards to apoptosis between 7.3 μM Sorafenib and 12.5 nM doxorubicin vs. control (α_adj = 0.006 for cytarbine and doxorubicine analyses; α_adj = 0.003 for RAD001 analyses). Results are displayed as mean +/- SD of three independent experiments.