Figure 3
Sorafenib inhibits cell cycle progression and metabolic activity. (A) Effects of Sorafenib on cell cycle distribution was determined by DNA staining with Propidium iodide and flow cytometry in SEM cell line. Sorafenib induces a reduction of cells in M and S phases. (B) Cell cycle arrest was confirmed by western blot 24 h after Sorafenib treatment in SEM and Jurkat cells. Membranes were incubated with the indicated antibodies against cell cycle regulator proteins. GAPDH was used as control for equal loading. Reduction of CDK4 and Cyclin D3 were detected at 7.3 μM Sorafenib in both cell lines. (C) SEM, RS4;11 and Jurkat cells were treated with the indicated concentration of Sorafenib for 48 h and metabolic activity were analyzed using WST-1 Assay. Metabolic activity measured by formazan dye is shown as a difference in absorbance measured at 450 nm and 620 nm (reference) wavelengths, respectively. Treatment with 7.3 μM of Sorafenib induced a statistically significant (*αadj = 0.017) inhibition of proliferation in SEM, RS4;11 and Jurkat cells, 0.73 μM Sorafenib in SEM cells as well. Results are displayed as mean +/- SD of three independent experiments.