Table 2 Primer sets
From: Canine and human gastrointestinal stromal tumors display similar mutations in c-KIT exon 11
Amplified Region | Forward | Reverse |
|---|---|---|
c-KIT exon 8 | 5'-CAGCAGTCTGACCTATGGC -3' | 5'-GCTCAGCTCCTGGACAGAAA-3' |
c-KIT exon 9 | 5'-GATTGATTGATTGATTTTCCTAG-3' | 5'-GCAGGCAGAGCCTAAACATC-3' |
c-KIT exon 11 | 5'-CATTTGTTCTCTACCCTAAGTGCT-3' | 5'-GTTCCCTAAAGTCATTGTTACACG-3' |
c-KIT exon 13 | 5'-CTGATTAAGTCGGATGCGGC-3' | 5'-CAAGCACTGTCGCAATGG-3' |
c-KIT exon 17 | 5'-ATAGCAGCATTCTCGTGTTG-3' | 5'-AACTAAAATCCTTCACTGGACTG-3' |
PDGFRA exon 12 | 5'-TTAATGGCTCTGATTGCTCAC-3' | 5'-CACCCAGTGCTCATAACCTC-3' |
PDGFRA exon 14 | 5'-ACTGGTTTTGGTTCCCACAG -3' | 5'-CAATGATTCGCAGCAACG-3' |
PDGFRA exon 18 | 5'-TAGCTCAGCCGTGGGTATG-3' | 5'-CACATGAGCAGAGATGTCAGG-3' |