Figure 3

c-Met inhibitor decreased proliferation of androgen receptor positive cells in vivo. LNCaP C4-2 cells were implanted into the renal capsule of male SCID mice supplemented with testosterone as described in the "Materials and Methods". At day 28, mice were sacrificed and the grafted tumor mass was identified. (A) Representative tumor tissue stained with hematoxylin and eosin, or analyzed by immunostaining with human AR or PSA antibody, respectively. (B) Tumor tissues were harvested and weighed from mice given water or daily oral gavage of PF-2341066 at 50 mg/kg. The difference of weight was analyzed by Student's t test (*P < 0.01). (C) Representative tumor tissue isolated from mice given water (left) or daily oral gavage of PF-2341066 at 50 mg/kg (right) and analyzed by immunostaining with Ki67 antibody. (D) Positive cells with Ki67 antibody were counted in more than 5 different fields, and the Ki67 index was measured as the number of the positive Ki67 cells relative to the number of total cells in two representative sections. There was a difference between the control and treated mice, *P < 0.01 (Student's t test).