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Figure 3 | BMC Cancer

Figure 3

From: Expression pattern of matrix metalloproteinases in human gynecological cancer cell lines

Figure 3

Analysis of the cell culture supernatants by gelatin zymography. The cell lines were first plated in serum-containing medium for 72 h. Afterwards, medium was replaced by serum-free DMEM/HamsF12 for an additional 48 h. Samples of conditioned medium were assayed for MMP-2 and MMP-9 by gelatin zymography. Gelatinolytic activity of pro and active MMP-2 and active MMP-9 are visible as a clear area on the gel, indicating where the gelatine has been digested.

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