Figure 5

Cells derived from PDGF-B-induced gliomas are tumorigenic also in the absence of NG2 expression. (A) Survival curves following transplantation into adult brains of PDGF-B-induced tumor cells derived from wild type (black line) or NG2-KO (green line) mice. The Log-rank test performed on the two survival distributions showed that there are no significant differences (Log-rank Test = N.S.). (B) Fluorescence image of a NG2-KO secondary tumor. (C-F) Coronal sections of secondary tumors derived from NG2-KO (C-D) or wild type (E-F) mice stained with DAPI (C, E) or hematoxylin and eosin (D, F). NT: non-tumor zone; T: highly cellularized tumor zone; Dashed contours highlight the tumor mass; arrows point pseudopalisade structures. Scale bars: 1 mm (B); 100 μm (C-F).