Methylation, mRNA expression and metastasis score. A) mRNA expression was plotted against methylation-score for each cell line to demonstrate the relevance of promoter methylation to mRNA expression. AKAP12 shows no significant correlation while SERPINB5 expression is significantly reduced in methylated cell lines. B) Cell lines with methylated SERPINB5 promoters have significantly lower metastatic potential (P < 0.05). MIAPaCa-2 is an outlier in the methylated group but has reduced metastatic ability compared to its strong invasive potential.