Bisulfite sequencing. Signals from BSP with direct sequencing. During direct sequencing cleaned up PCR products without prior cloning were used, the sequenced signal therefore represents average methylation across the entire PCR template. A) 3 distinct signal patterns were identified: Unmethylated cytosines with a T-signal at the CpG site, heterogeneously methylated CpGs with superimposing C- and T-signals and methylated CpGs with a C-signal. B) BSP-analysis of promoter methylation for AKAP12 and SERPINB5. Each box represents a sequenced CpG-dinucleotide.