Figure 2

Effects of miR-339-5p on breast cancer cells. A. Regulation of breast cancer cell growth by miR-339-5p. MCF-7 cells were grown and transiently transfected with miR-339-5p antisense oligonucleotide or scrambled sequence oligonucleotide as negative control for 4 days, and cell viability was analyzed using a kit. The experiments were in triplicate and repeated twice. B. Wound-healing assay. MCF-7 cells were grown and transiently transfected with miR-339-5p antisense oligonucleotides or scrambled sequence oligonucleotide as negative control for 3 days and subjected to the wound-healing assay. Magnification: ×100. C. Transwell migration and Matrigel invasion assays. MCF-7 cells were grown and transiently transfected with miR-339-5p antisense oligonucleotides or scrambled sequence oligonucleotide as negative control for 2 days and subjected to migration and invasion assays. Representative photographs (upper) and quantification (lower) are shown. Magnification: ×200.