|
Genotype
|
9cRA
|
Animals-No
|
PIN
|
P
|
Tumors*
No
|
|---|
| | |
at start
|
at end
|
Incidence
|
Frequency
| | |
|---|
|
p27+/+
|
0
|
20
|
15
|
13
|
0.13 ± 0.3
| |
2
|
|
p27+/+
|
+
|
20
|
15
|
13
|
0.13 ± 0.3
|
ns
|
1
|
|
p27+/-
|
0
|
20
|
10
|
40
|
0.7 ± 0.3
| |
4
|
|
p27+/-
|
+
|
20
|
10
|
60
|
1.3 ± 1.1
|
ns
|
3
|
|
p27-/-
|
0
|
20
|
10
|
80
|
2.3 ± 1.2
| |
5
|
|
p27-/-
|
+
|
20
|
8
|
87
|
1.7 ± 1.0
|
ns
|
5
|
- Animals were implanted with a testosterone pellet at the age of 2 months and 6 days later MNU was injected ip to initiate the neoplastic process. Four days after carcinogen administration an estradiol pellet was sc implanted. %, incidence of tumors among control and treated animals; No: multiplicity of PIN per treated animal; ns, not significant (p > 0.05). To determine the incidence (%) and frequency of PIN in DLP, 4 μm thick longitudinally paraffin sections through the urethral part of the prostate were cut stepwise at three separate levels 30 μm apart.
