Aggressive mammary carcinoma progression in Nrf2 knockout mice treated with 7,12-dimethylbenz[a]anthracene

BMC Cancer

Table 3 Comparison of WT vs KO mice Quantitative real-time pcr array: mouse stress response pathway

Rank	Well	Gene Name		Fold Change
1	A10	Casp3	caspase 3, apoptosis related cysteine protease	-2.69
2	H02	Cdh1	cadherin 1	2.91
3	H12	Txnrd2	thioredoxin reductase 2, mitochondrial	3.53
4	A03	Mmp9	matrix metallopeptidase 9	-6.83
5	F03	Gpx3	glutathione peroxidase 3	13.2
6	C03	Hspb2	heat shock protein 2	1.66
7	G03	Gpx7	glutathione peroxidase 7	3.09
8	D06	Mmp2	matrix metallopeptidase 2	1.65
9	A12	Dnajc5	DnaJ (Hsp40) homolog, subfamily C, member 5	-1.73
10	D05	Aprt	adenine phosphoribosyl transferase	-1.86
11	B06	Bcl2l1	BCL2-like 1	-1.59
12	F10	Myc	myelocytomatosis oncogene	-1.60
13	F06	Sod1	superoxide dismutase 1, soluble	-1.74
14	E12	Rad9	RAD9 homolog (S. pombe)	-1.86
15	C04	Trp53	transformation related protein 53	-1.82
16	H08	Xpa	xeroderma pigmentosum, complementation grp A	1.49
17	F07	Aoc3	amine oxidase, copper containing 3	2.13
18	H10	Pklr	pyruvate kinase liver and red blood cell	1.96

Global Pattern Recongition TM (GPR) Results for Mouse Stress Response 96 StellARray™. Four WT mammary carcinomas (125T3, 135T1, 191T3, and 139T6) were compared to four KO mammary carcinomas (183T1, 165T1, 155T1, and 172T1). The selection of mammary carcinomas for the real-time pcr experiment was based on the date of necropsy so that the mice would be fairly well age-matched. The average days on the study for the WT compared to Nrf2 KO mice were 166 vs. 170, respectively. Also, these tumors were strictly mammary adenocarcinomas, not mixed with lymphoma or adenoacanthoma. P-values ranged from 0.11 - 0.24, and so these results are considered "leads" not statistically significant.

ISSN: 1471-2407