Figure 5

Immunohistochemical expression analysis in ERBB2 -amplified tumors. Two exemples of IHC profile of ERBB2-amplified cases. The IHC profiles show in ERBB2-amplified ERBB2-positive (a-f) an ERBB2 expression defined as 3+ with Herceptest (a) and TAB250 (b) as well as a cytoplasmic positivity of pERBB2 (c) a cell membrane positivity of IFG1R (d) and an absence of FOXA1 (e) and EGFR (f) expression. The IHC profiles show in ERBB2-amplified ERBB2-negative (g-m) an ERBB2 expression defined as 0+ with Herceptest (h) and TAB250 (i) as well as a weak cytoplasmic positivity of pERBB2 (j) no positivity of cell membrane of IFG1R (k), a strong nuclear expression of FOXA1 (l) and an absence of EGFR (m).