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Table 1 CD44 methylation status, mRNA and protein expression in lymphoma cell lines

From: Epigenetic regulation of CD44in Hodgkin and non-Hodgkin lymphoma

subtype

cell line

MS-MLPA

MSP

CD44mRNA

Aza induction of CD44mRNA

CD44 protein

HL

KM-H2

M

M/U

positive

-

positive

 

L-428

M

U

positive

n.d.

positive

 

L-1236

U

U

positive

n.d.

n.d.

 

SUP-HD1

U

U

positive

n.d.

positive

ALCL

DEL

U

M/U

positive

n.d.

positive

 

KARPAS-299

M

M/U

negative

+++

negative

BL

CA-46

M

M

negative

+++

negative

 

DAUDI

M

M/U

negative

+

n.d.

 

EB-1

M

M

negative

++

negative

 

RAJI

M

M

negative

+++

negative

FL

DOGUM

U

U

negative

-

n.d.

 

DOHH-2

M

M/U

positive

-

n.d.

 

SC-1

M

M/U

negative

+++

negative

 

WSU-FSCCL

M

M

negative

++

n.d.

DLBCL

DB

M

M/U

negative

+

n.d.

 

HT

M

M/U

negative

++

negative

 

SU-DHL-4

M

M/U

negative

n.d.

negative

 

WSU-DLCL2

U

U

negative

-

negative

MCL

GRANTA-519

U

U

positive

-

positive

 

HBL-2

U

M/U

positive

n.d.

positive

 

JEKO-1

M

U

positive

-

positive

 

REC-1

U

U

positive

-

positive

  1. Gene expression and induction of CD44 mRNA transcription were analyzed by qRT-PCR. Aza induction was tested after incubation of cells for 3 days with 5 μM Aza. CD44 protein expression was analyzed by flow cytometry. MS-MLPA and MSP results: M: methylated, U: unmethylated; fold of Aza induction: + > 3 ×, ++ > 30 ×, +++ > 150 ×, - = no change; n.d.: not determined.