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Table 2 PCR Primers used for FLT3 ITD Backtracking on Guthrie card DNA: Northern California Childhood Leukemia Study

From: FLT3mutation incidence and timing of origin in a population case series of pediatric leukemia

Primer Name

Primer Sequences‡ (5'(3')

ITD-R2

AGACAAATGGTGAGTACGTGCA

ITD-4-F

ATATGATCTCtccgagggcc

ITD-87-F

TATGAATATGAgTTCAGAGAATATG

ITD-104-F

GAGTTTCCcctcgggaag

ITD-261-F

AGAGAATATGAGACCGGCTC

ITD-1073-F*

GAGTACTTCccCAGAGAATATG

ITD-1148-F

AGAGAATATGAgTACGTTGATTTC

  1. ‡ bold, WT sequence; underlined, ITD; lower case, N nucleotides
  2. *1073 had an 11 bp deletion with a 2 bp insertion, giving an overall deletion of 9 bp.
  3. ITD-R2 was used as the reverse primer for all the backtracking reactions.