Figure 5
Effect of TGZ on tumor growth and pulmonary metastasis. (A-C) After 21 days of TGZ treatment, the body weight (A), lung weight (B), and tumor weight (C) were measured. Body weight was measured after the excision of the tumor. Values given are the means ± SE for six mice in the normal group, thirteen mice in the control group, and fourteen mice in the TGZ group. *p < 0.05, **p < 0.01 (compared with the normal group). ***p < 0.05 (compared with the control group). (D) Immunohistochemistry for PCNA was performed using the primary tumor sections. The values given in photographs show the PCNA-labeling index and are the means ± SE for five determinations in the control group and ten determinations in the TGZ group. *p < 0.01 (compared with the control group). (E) HE staining of the lung sections was performed. Arrow: metastatic tumors. (F) The proportional area of pulmonary metastasis was calculated by dividing the sum of the areas of lung metastasis by the entire area of lung tissue. Values given are the means ± SE for thirteen mice in the control group and fourteen mice in the TGZ group. Magnification: ×100. *p < 0.01 (compared with the control group).