Effect of topical treatments including steroid and barrier film spray on pro-inflammatory cytokines in skin and subcutaneous tissues after irradiation by Western blotting analysis. Expressions of COX-2, IL-6, TGF-β1 and MCP-1protein in irradiated murine skin and subcutaneous tissue with or without topical treatment including steroid cream and barrier film spray. Proteins were extracted form murine tissues (a) 24 h; (b) 7 days after irradiation. (C, control; RT, irradiation; RT+S, steroid cream plus irradiation; RT+F, barrier film spray plus irradiation). Triplicate experiments were performed for the analysis. Twenty- four hours after irradiation, topical steroid cream significantly attenuated the increase of COX-2, IL-6, TGF-β1 and MCP-1 in cutaneous tissues, but barrier film spray had no effect Furthermore, both topical treatments alleviated the RT-induced response 7 days after irradiation.