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Table 2 Primer sequences and amplicon sizes for selected genes.

From: Expression profiling in canine osteosarcoma: identification of biomarkers and pathways associated with outcome

Primer Sequence (5' to 3') Size of Amplicon
HPRT1 S TGC TCG AGA TGT GAT GAA GG 191 bp
HPRT1 AS TCC CCT GTT GAC TGG TCA TT  
ADHFE1 S CCA ACA GTG GCT TCG ATG TGC TTT 104 bp
ADHFE1 AS TGC TGG CCG AGT GAT AGG ATT TGA  
AGTR1 S TGA CTT TGC CAC TAT GGG CTG TCT 178 bp
AGTR1 AS AGG CGG GAC TTC ATT GGA TGA ACA  
CCDC3 S TGA ACC AGA AGC TCA GCG AGA AGT 162 bp
CCDC3 AS TAG ATT CCC TGG CAA GAG GCA ACA  
DHH S ACA ACC CGG ACA TCA TCT TCA AGG 109 bp
DHH AS ATG TTC ATC ACC GCA ATG GCC AAG  
FBP1 S TCC TGT ACC CAG CGA ACA AGA AGA 89 bp
FBP1 AS TGC CTT CTC CAT GAT GTA GGC CAT  
IGF2 S TCG TGG AAG AGT GCT GTT TCC GTA 154 bp
IGF2 AS TCG TAT TGG AAG AAC TTG CCC ACG  
IMP1 S TTG CAG AAT TTG ACA GCG GCT GAG 118 bp
IMP1 AS TTT GGT GCA GCT GCT TAA CTT GGG  
NDRG2 S ATA AGT CTT GCT TCC AGC CGC TCT 183 bp
NDRG2 AS TCA GGT ACT GCA GAA TGC AAG GGA  
PTCH2 S CAT ATT CCT GCT GGC ACA TGC CTT 229 bp
PTCH2 AS GAA GAC AAG CAT CAC GGC TGC AAA  
SCN1B S TCG TGG CAG AGA TGG TTT ACT GCT 121 bp
SCNIB AS ACA CCC GTA CAG TTC TCC TTG CTT  
SMO S TGG TGC TCA TTG TGG GAG GTT ACT 210 bp
SMO AS ACT CAG CCT GGT TGA AGA AGT CGT  
  1. S = sense, AS = antisense, bp = base pairs