qRT-PCR validation of genes selected from fold change analysis of microarray data. Expression represented as 2(-ΔCt) for eight genes selected from fold change analysis of gene array data that were significant on qRT-PCR validation with 20 samples. Higher values indicate higher expression between cohorts and among genes. Fold change and p-values calculated via the REST2009 method. Error bars represent standard error. Insulin-like growth factor II (IGF2, A), alcohol dehydrogenase, iron containing 1 (ADHFE1, B), coiled-coil domain containing 3 (CCDC3, C), sodium channel, voltage-gated, type I, beta (SCN1B, D), angiotensin II receptor, type 1 (AGTR1, E), n-myc downstream-regulated gene family member 2 (NDRG2, F), fructose-1,6-bisphosphatase 1 (FBP1, G), and IGF2 mRNA binding protein 1 (IMP1, H).