Figure 4

The amount of IFN-γ produced by (a) DCs treated with different concentrations of TRF (2 to 15 μg/ml) and (b) splenocytes treated with different concentrations of TRF (2 to 15 μg/ml) (c) DCs co-cultured with tumour lysate from 4T1 cells in the presence of 8 μg/ml TRF for 72 h. Following 72 hours of culture, the supernatants of control, TRF alone, DC with tumour lysate from 4T1 cells (DC+TL), DC with 8 ug/ml of TRF (DC+TRF), DC with tumour lysate from 4T1 cells and 8 μg/ml TRF (DC+TL+TRF) were harvested. The amount of IFN-γ in the supernatant was determined using ELISA. The amount of IL-12 produced by (d) DCs treated with different concentrations of TRF (2 to 15 μg/ml) and (e) splenocytes treated with different concentrations of TRF (2 to 15 μg/ml) (f) DCs co-culture with tumour lysate (DC+TL), in the presence of 8 ug/ml TRF (DC+TL+TRF), DC alone in the presence of ug/ml TRF (DC+TRF), TRF alone and control for 72 h.