Figure 2From: Overexpression of sphingosine kinase 1 is associated with salivary gland carcinoma progression and might be a novel predictive marker for adjuvant therapy Expression of SPHK1 is elevated in human primary SGC tissues compared with adjacent noncancerous tissues. (A) Expression of SPHK1 mRNA in each of the primary SGC tissues (T) and SGC-adjacent noncancerous tissues (ANT) in the same patient, determined by real-time PCR. (B and C) Expression (B) and Quantification (C) of SPHK1 protein in each of the primary SGC tissues (T) and SGC-adjacent noncancerous tissues (ANT) in the same patient, determined by western blotting. (D) Expression of SPHK1 protein in each of the primary SGC tissues (T) and SGC-adjacent noncancerous tissues (ANT) in the same patient, determined by immunohistochemistry (IHC). Data in A to D was performed three independent times with similar results. *, P < 0.05.Back to article page