Figure 3

Examples of growth factor and ErbB2-dependent differential gene expression. Plots of relative gene expression (averaged (n = 4) normalised fluorescence intensity) versus time are shown for 20 genes. Data for both microarray clones are shown for IGFBP3. Genes showing a change in expression following growth factor treatment and significantly different between the cell lines were: IGFBP3_1 (1.18-fold increase in HB4a versus 1.3-fold decrease in C3.6 with EGF (0 to 4 hr); MYC (1.05-fold decrease in HB4a versus 3.09-fold increase in C3.6 with HRG (0 to 4 hr); MAP2K3 (1.39-fold increase in HB4a versus 2.49-fold increase in C3.6 with HRG (0 to 4 hr); BCAR3 (1.52-fold increase in HB4a versus 3.18-fold increase in C3.6 with HRG (0 to 4 hr); ZYX (1.05-fold decrease in HB4a versus 3.17-fold increase in C3.6 with HRG (0 to 4 hr); PLAT2 (1.18-fold increase in HB4a versus 2.13-fold increase in C3.6 with EGF (0 to 4 hr) and 1.01-fold increase in HB4a versus 2.76-fold increase in C3.6 with HRG (0 to 4 hr); TNFAIP3 (4.98-fold increase in HB4a versus 7.74-fold increase in C3.6 with EGF (0 to 4 hr); GADD45A (1.26-fold increase in HB4a versus 2.82-fold increase in C3.6 with HRG (0 to 4 hr); S100A2 (2.88-fold increase in HB4a versus 1.02-fold increase in C3.6 with EGF (0 to 4 hr); ERBB2 (3.13-fold decrease in HB4a versus 1.48-fold increase in C3.6 with HRG (0 to 4 hr); ZNF236 (4.85-fold increase in HB4a versus 2.83-fold increase in C3.6 with HRG (0 to 4 hr).